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Novus試劑盒標準曲線做不好的原因做出分析
發(fā)布時間： 2022-06-21 點擊次數(shù)： 1001次Novus試劑盒內(nèi)包含了實驗所需的所有試劑和相關組份。簡單快捷的方案設計和優(yōu)化的試劑配比，為全球科研工作者提供實驗分析方案。該試劑盒內(nèi)含有分析所需的所有必需試劑，操作簡單便捷；試劑盒內(nèi)組分經(jīng)實驗優(yōu)化，確保更高的檢測靈敏度。Novus試劑盒的實驗原理：試劑盒的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。在測定時，受檢標本與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質(zhì)分開。再加入酶標記的抗原或抗體，也通過反應而結(jié)合在固相載體上。此時固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例。加入酶反應的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關，故可根據(jù)呈色的深淺進行定性或定量分析。Novus試劑盒標準曲線做不好的原因做出分析：1、剛加完顯色劑時梯度明顯：顯色液可能是從高濃度向低濃度孔加的；2、酶濃度太高，導致最終顯色結(jié)果都是高的；3、包被-酶標系統(tǒng)不匹配或許酶標的非特異反應太強，或許酶標儀讀數(shù)規(guī)劃不夠；4、樣本中有可以催化底物的物質(zhì)，沒洗下來；5、建議：包被、酶標重新做梯度，先用較低的濃度把梯度探究好，然后再優(yōu)化靈敏度，最終調(diào)出所需的靈敏度、線性規(guī)劃；6、有條件的話，可以把酶免的蛋白系統(tǒng)移植到板式化學發(fā)光上，加完底物三分鐘讀數(shù)；7、蛋白系統(tǒng)靈敏度夠的話，顯色十分鐘就差不多了；8、酶是自己標的這種情況的，HRP比例不要太高。
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